Detection of blaSHV, blaTEM and bla CTX-M among ‎Urinary Tract Infection Escherichia coli isolates

Authors

  • Mun,im Radwan Ali Departme of science -Collage of basic education- Al-Mustansiryah University‎
  • Maha Mekhlef Kahdim Department of biology -Collage of science- Al-Mustansiryah University‎
  • Shahbaa Hamid Majeed Departme of science -Collage of basic education- Al-Mustansiryah University‎

Keywords:

blaSHV, blaTEM and blaCTX-M, PCR assay‎, Urinary tract infection‎, E.coli

Abstract

One hundred fifty urine samples were collected from urinary tract infection patients. We obtained 40 isolates of Escherichia coli among these clinical samples. A proportion of UTI in females 93 (62%) is higher than that in males 57 (38%). All locally isolates are multi-resistant, the highest rate of resistance was seen with Amoxicillin/clavulanic acid, and Cloxacillin. The results showed high percentage 14(35%) of class C among locally isolates , while only 4(10%) are able to produce Class A ESβL , on the other hand  (7/40) and (8/40) were ESβL neither Class A nor Class C Present and ESβL absent respectively , while among the 40 isolates only 7 isolates were Non-Typable. Three types of  β-lactamases include ESβLs genes (blaSHV, blaTEM, blaCTX-M) by the use of uniplex PCR assay. The blaSHV was detected in 1 isolates (2.5%) of the UPEC isolates, while for the blaTEM gene the result showed that in 27 detected isolates (67.5%), there is 23 isolates (57.5%) which indicates the prevalence of  blaCTX-M gene. The results showed the Co-existence  of blaTEM, blaCTX-M in 30% of isolates, while one isolate  blaSHV, blaTEM, blaCTX-M  in their genome component

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Published

2017-11-30

How to Cite

[1]
M. R. Ali, M. M. Kahdim, and S. H. Majeed, “Detection of blaSHV, blaTEM and bla CTX-M among ‎Urinary Tract Infection Escherichia coli isolates”, JUBPAS, vol. 25, no. 5, pp. 1700-1707, Nov. 2017.

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Articles